High-Resolution Imaging and Automated Analysis for Veterinary Lateral Flow Immunoassays: Evaluation of the LF Reader Plus System

The Lateral Flow (LF) Reader Plus integrates high-resolution imaging and automated analysis algorithms for veterinary lateral flow immunoassays (LFIAs). This study aims to evaluate its functional reliability and application scope through systematic testing on diverse animal specimens. Key functions (Setting, Records, Read, Upload, Print) and auxiliary features (self-test protocols, calibration routines) were validated. Operational workflows showed 100% reproducibility across 1200 tests. Compared with traditional devices, the reader demonstrates a 40% improvement in analysis speed and 35% enhancement in quantitative precision, supported by real-time thermal printing documentation. While assay-specific compatibility limitations exist, the device's clinical utility is underscored by its ability to deliver rapid, data-driven diagnostics. This study highlights its role as an efficient tool for veterinary point-of-care testing, advocating for expanded compatibility development and standardized user training.