Aminated cinnamic acid analogs as dual polarity matrices for high spatial resolution MALDI imaging mass spectrometry
Improved sensitivity and absorption efficiency at 355 nm allow for 5 μm pixel size MALDI IMS without oversampling while maintaining high S/N on commercial mass spectrometry platforms. Performing MALDI experiments at reduced laser energies minimizes tissue damage, enabling advanced multimodal MALDI IMS studies to be performed on single tissue sections. Comparisons and optimized MALDI IMS methods were performed on murine tissues and human kidney samples as part of the Human Biomolecular Atlas Program.
Authors
- Katerina Djambazova (ORCID: https://orcid.org/0000-0002-2680-9014)
- Raf Van de Plas (ORCID: https://orcid.org/0000-0002-2232-7130)
- Madeline E. Colley (ORCID: https://orcid.org/0000-0002-9515-3493)
- Martin Dufresne (ORCID: https://orcid.org/0000-0002-1731-3666)
- Lukasz G. Migas (ORCID: https://orcid.org/0000-0002-1884-6405)
- Jeffrey M. Spraggins (ORCID: https://orcid.org/0000-0001-9198-5498)
Institutions
- Vanderbilt University (US)
- Delft University of Technology (NL)
Publication Details
- Journal
- Analytica Chimica Acta
- Published
- 2025-07-16
- DOI
- https://doi.org/10.1016/j.aca.2025.344423
- Citations
- 6
- Primary Topic
- Mass Spectrometry Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 4.54
Funders
- National Science Foundation
- Silicon Valley Community Foundation
- Chan Zuckerberg Initiative
- National Institutes of Health
- National Institute on Aging
- National Institute of Diabetes and Digestive and Kidney Diseases