The effect of physical exercise on the proliferative and enzymatic potential of the liver and under conditions of correction with mildronate

The purpose of the study is the effect of mildronate on the proliferation of hepatocytes and on the activity of liver enzymes in rats during physical activity of varying intensity. Material and methods. 84 white male rats weighing 240 grams were used as experimental animals. The animals were divided into three groups. The experiment was carried out in two stages. In the first stage, the rats were given loads of varying intensity: the first group of rats was given a light physical load, for which they were placed in a bath with a water temperature of 29-32˚C in which the animals swam for 15 minutes. The rats of the second group spent 30 minutes in the bath; this load was considered to be of moderate severity. To simulate heavy physical activity (third group), the animals swam in the bath until they began to lose strength and drown. This usually occurred 55-59 minutes after the animals were in the water. After being removed from the bath, the animals of this group, unlike the animals of the first two groups, were lethargic, lay down for some time, and did not eat. Animals of all groups underwent 10 sessions of water loading, after which they were taken out of the experiment immediately after the last session (7 animals per group) and 30 days after the end of the experiment (7 animals per group) using zoletil anesthesia at the rate of 5 mg per 100 g in accordance with the International Rules for Work with Experimental Animals. At the second stage, another group of rats, also divided into three groups, received a similar load of varying intensity, however, throughout the entire experiment (10 days), oral administration of mildronate, 3-(2,2,2-trimethylhydrazine) propionate at a dose of 100 mg was observed daily. / kg. Control animals (n=7) were white rats weighing 240 grams. After removing the liver, pieces measuring 1x1 cm were cut out from it, which were fixed in 10% neutral formaldehyde and embedded in paraffin; The resulting serial sections were stained with hematoxylin and eosin and van Gieson. Binucleate hepatocytes were counted per 7000 cells. SDH, NADH, NADFH, acid and alkaline phosphatases were determined histochemically in hepatocytes. Statistical analysis was carried out using the Statistica-6 program. Student’s t-test was used to analyze differences between samples. Research results. The study found that the use of mildronate when rats performed physical activity significantly changes the situation: the proliferative activity of hepatocytes increases when rats perform light and moderate exercise, occurring against the background of increased activity of redox enzymes. When rats perform heavy workloads, the use of mildronate supports the reproductive potency of hepatocytes and the normal enzymatic characteristics of the liver. Conclusion. The study shows that the use of mildronate is not only desirable when animals perform heavy physical activity, but also, apparently, is necessary for health reasons.