Transcription level of genes of the glutathione-S-transferase system in the liver of rats under long-term exposure to acrylamide with prophylactic administration of hydroxymethyluracil complex compounds

The purpose of the study was to assess the level of transcriptional activity of the Gstt1 and Gstm1 genes in the liver of rats under long-term exposure to acrylamide against the background of preventive correction with hydroxymethyluracil complex compounds. Materials and methods. The experiment was carried out on outbred male rats with oral administration of acrylamide at a dose of 5 mg/kg and complex compounds of hydroxymethyluracil with ascorbic acid, sodium succinate and acetylcysteine in a prophylactic regimen for three months. The study of gene transcription in liver tissue samples was carried out using real-time polymerase chain reaction using oligonucleotide specific primers and the intercalating dye SYBR Green. The Gapdh gene was used as a reference gene. For statistical analysis of the results, the IBM SPSS Statistics 21 application package was used. Results. In our study, the expression of the Gstm1 and Gstt1 genes decreased after 1.5 months of exposure to acrylamide in animal liver tissue samples. The introduction of prophylactic drugs contributed to an increase in its level: to a greater extent, a complex compound of WMU with sodium succinate, which was statistically significant, and to a lesser extent, complex compounds of WMU with ascorbic acid and acetylcysteine. After 3 months, differences between groups were less noticeable for these genes. Conclusion. With long-term exposure to acrylamide, the most significant changes in gene expression in rat liver tissue were observed at 1.5 months, compared with data at the end of the experiment. At the same period of the study, complex compounds of oxymethyluracil have a certain corrective effect on the transcriptional activity of both the Gstt1 gene and the Gstm1 gene. To make a final judgment about the protective properties of the complex compounds being studied, further research is needed to analyze changes in other indicators of the health status of laboratory animals.