“A Novel Dual-Extract Green Synthesis of Silver Nanoparticles Using Tulasi And Sandalwood: NMR-Assisted Characterization and Antimicrobial Assessment”

Abstract: This study explores a green synthesis method for silver nanoparticles (AgNPs) using aqueous extracts of Tulasi (Ocimum sanctum) and Sandalwood (Santalum album). The extracts provide phytochemicals that act as reducing and stabilizing agents. The synthesized AgNPs were characterized using UV–Vis spectroscopy, FTIR, XRD, NMR, SEM, TEM, and zeta potential analysis. The AgNPs exhibited strong antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans, demonstrating the potential of these natural resources in nanomaterial development. This study uniquely integrates Tulasi and Sandalwood extracts for the first time in silver nanoparticle synthesis, with NMR characterization rarely reported in similar green synthesis approaches.