Agromyces chitinivorans sp. nov., Agromyces litoreus sp. nov. and Agromyces zhanjiangensis sp. nov., three novel chitin-degrading actinobacteria isolated from a mudflat sediment
Three strains of actinobacteria, designated as SYSU T00194<sup>T</sup>, SYSU T00242<sup>T</sup> and SYSU T00266<sup>T</sup>, were isolated from tidal flat sediment samples in Zhanjiang, PR China. The taxonomic position of three isolates was determined using polyphasic taxonomic analysis and phylogenomic analysis. Phylogenetic analysis and 16S rRNA gene sequence comparisons indicated that strain SYSU T00194<sup>T</sup> formed a distinct clade with <i>Agromyces rhizosphaerae</i> IFO 16236<sup>T</sup> and shared a sequence identity value of 97.9%. As well as strains SYSU T00242<sup>T</sup> and SYSU T00266<sup>T</sup>, which formed a distinct clade with <i>Agromyces aurantiacus</i> CGMCC 4.1717<sup>T</sup>, exhibiting sequence identity values of 97.7 and 97.6%, respectively. Furthermore, they also exhibited a closer phylogenetic relationship with <i>Agromyces luteolus</i> IFO 16235<sup>T</sup>, as determined by phylogenomic analysis, with average nucleotide identity values of 86.1 and 91.1%, respectively. These novel isolates could be distinguished from species in the genus <i>Agromyces</i> by their distinct phenotypic and genotypic characteristics. The cells of three novel strains were aerobic, Gram-stain-positive and short rod-shaped. Optimal growth conditions of strains SYSU T00194<sup>T</sup>, SYSU T00242<sup>T</sup> and SYSU T00266<sup>T</sup> occurred on the marine agar 2216 with pH 8.0 at 37 °C. In addition, the major fatty acids of SYSU T00194<sup>T</sup>, SYSU T00242<sup>T</sup> and SYSU T00266<sup>T</sup> were iso-C<sub>16:0</sub>, anteiso-C<sub>15:0</sub> and anteiso-C<sub>17:0</sub>. The major polar lipids included diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and glycolipid (GL). According to the phenotypic, genotypic and phylogenetic data, strains SYSU T00194<sup>T</sup>, SYSU T00242<sup>T</sup> and SYSU T00266<sup>T</sup> represent three novel species of the genus <i>Agromyces</i>, for which the names <i>Agromyces chitinivorans</i> sp. nov., <i>Agromyces litoreus</i> sp. nov. and <i>Agromyces zhanjiangensis</i> sp. nov. are proposed, respectively. The type strains of the proposed three novel isolates are SYSU T00194<sup>T</sup> (=MCCC 1K09402<sup>T</sup>=KCTC 59364<sup>T</sup>), SYSU T00242<sup>T</sup> (=GDMCC 1.3836<sup>T</sup>=KCTC 44948<sup>T</sup>) and SYSU T00266<sup>T</sup> (=GDMCC 1.3835<sup>T</sup>=KCTC 49949<sup>T</sup>), respectively.
Authors
- Dildar Wu
- Yong-Hong Liu (ORCID: https://orcid.org/0000-0001-5409-803X)
- Ting‐Ting She
- Wen‐Jun Li (ORCID: https://orcid.org/0000-0002-1233-736X)
- Yao Zhang (ORCID: https://orcid.org/0000-0001-5841-225X)
- Chun-Yan Lu
- Lei Gao (ORCID: https://orcid.org/0000-0002-6059-5347)
- Bao‐Zhu Fang (ORCID: https://orcid.org/0000-0003-1263-9664)
- Qing-Yu Xu
- Yue-Heng Chen
- Xinyao Li (ORCID: https://orcid.org/0000-0001-8895-3858)
Institutions
- Chinese Academy of Sciences (CN)
- Guangdong University of Education (CN)
- Institute of Ecology and Geography (MD)
- Xinjiang Institute of Ecology and Geography (CN)
- Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (CN)
- University of Chinese Academy of Sciences (CN)
- Guangxi Academy of Sciences (CN)
Publication Details
- Journal
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
- Published
- 2025-07-14
- DOI
- https://doi.org/10.1099/ijsem.0.006831
- Citations
- 1
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Natural Science Foundation of Xinjiang Province