Effect of Thymoquinone and Butylated Hydroxytoluene on Behavior and Oxidative Stress Level in Rats Chronically Exposed to Ethanol

The objective of this study is to examine the antioxidant activity of Thymoquınone (TQ) and Butylated Hydroxytoluene (BHT) and its effect on behavioral tests in rats chronically exposed to ethanol. The experimental groups were determined as Control, Sham, Ethanol, Ethanol+BHT, Ethanol+TQ and Ethanol+BHT+TQ. In order to create a chronic alcohol exposure model in rats, 7 g/kg ethanol was given for 4 weeks. BHT and TQ were given at a dose of 10 mg/kg for 4 weeks. Glutathione, malondylaldehyde and total nitric oxide levels were analyzed to evaluate oxidative stress in brain, stomach, liver and kidney tissue. Open field test and forced swim test were used to examine anxiety disorder and depression-like behaviors in rats. Compared with the control group, ethanol exposure increased malondylaldehyde and total nitric oxide levels and decreased glutathione levels in all tissues (P