Evaluating the hepatoprotective effects of polyherbal extracts a comprehensive study on liver function and health in rat

ABSTRACT Background: this study evaluates the hepatoprotective effects of a polyherbal extract comprising Ziziphus spina-christi, Trigonella foenum-graecum, and Nigella sativa (ZTN) on liver function and health in male and female rats. The experiment involved administering two different doses (100mg/kg and 250mg/kg) of the polyherbal extract to groups of rats over a 14-day period, with a control group receiving water. Liver enzyme levels (ALT, AST, ALP) were measured to assess liver function, and histopathological examinations were conducted to evaluate liver tissue integrity. The results indicated that the polyherbal extract had a dose-dependent hepatoprotective effect in both male and female rats. Rats treated with the polyherbal extract showed significant reductions in liver enzyme levels compared to the control group, suggesting improved liver function. Histopathological analysis revealed reduced hepatocyte degeneration and inflammation in treated groups, with higher doses providing better protection. Comparative analysis between sexes showed similar hepatoprotective effects, indicating that the polyherbal extract is equally effective in male and female rats. The findings support the potential of polyherbal formulations as effective hepatoprotective agents, warranting further research to explore their mechanisms and efficacy in clinical settings. These findings contribute to the growing body of evidence supporting the use of polyherbal formulations for liver health and suggest promising avenues for developing effective treatments for liver diseases.