Characterization and Multi-Omics Basis of Biofilm Formation by Lactiplantibacillus plantarum

Lactiplantibacillus plantarum is a kind of common lactic acid bacteria, which plays an important role in the production of fermented foods. In general, the formation of biofilm is conducive to the adaptability of cells in the face of fierce competition and an increasingly harsh fermentation environment. In this work, optimum conditions for the formation of biofilm by L. plantarum were investigated, and scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) analysis showed the morphology of biofilm cells and 3D architecture of biofilm under different conditions, respectively. In addition, cells in the biofilms showed higher cell viability under heat stress, acid stress, and oxidative stress compared with planktonic cells. RNA-seq technology and TMT-based proteomic technology were employed to reveal the differential expression of profiles between biofilm cells and planktonic cells. The shelter provided by biofilm and the differential expression of genes and proteins involved in PTS, the TCA cycle, alanine, and teichoic acid biosynthesis may be involved in the formation of biofilm cells. The results presented in this study will help to understand the formation of biofilms in L. plantarum and regulate the industrial performance of cells in the food industry.