CMOS-fabricated dielectrophoretic chip with embedded 3D TiN nano-electrode arrays for sperm capture and sperm damage reduction

The results showed that square waves improved capture efficiency by approximately10% and confirmed that the capture space must exceed half the sperm length to enhance efficiency. When operated under a 20 Vpp square wave, the chip only generated a temperature rise of 1.7°C, causing no significant damage to the sperms and no notable decrease in viability. Under optimal conditions, the capture efficiencies for boar and bovine sperms reached 65.54% ± 1.07% and 63.25%, respectively. Overall, the results demonstrate that this chip offers high throughput, low Joule heating interference, and good species adaptability, showing potential for use in dynamic cell capture and high-throughput analysis.