Antidiabetic and Anti-inflammatory Properties of N-Phenyl Piperazine Derivatives through In Vitro Evaluations and Molecular Docking Studies

N-phenyl Piperazine derivatives have increasingly been recognized for their profound medicinal properties. This study embarks on the synthesis and study of such derivatives, specifically focusing on their in vitro α-amylase inhibitory and anti-inflammatory potential. Employing in silico molecular docking strategies, we assessed the interactions of these derivatives with the α-amylase enzyme (1HNY.pdb). Compounds P6, P7, and P22 emerged with commendable docking scores as -8.44, -8.37, and -8.49 kcal/mol, prompting their synthesis and assessment of in vitro α-amylase activity assessment. Among the studied compounds, P7 demonstrated robust inhibitory effects against both α- amylase and inflammation. Complementing the docking studies, this comprehensive investigation underscores the potential of N-phenyl Piperazine derivatives as potent bioactive molecules.