Sequential pathology of fowl adenovirus serotype 11 in experimentally infected chicken

The present study was undertaken to find out the sequential pathology of fowl adenovirus in experimentally infected chicken. To assess the gross and histopathology, experimental infection of ninety-eight numbers of one-day- old chicks were inoculated with fowl adenovirus (FAdV) serotype 11 field isolate at the rate of 0.5 ml of 106.5/TCID 50 per ml both oral and intramuscular (I/M) route except control group, followed by PCR confirmation and sequencing. Six number of birds from each treatment groups and two number of birds from control groups were sacrificed with 3, 5, 7, 10, 14, 17 and 21st days post infection (dpi) and detailed post mortem examination was conducted. Tissue samples viz., bursa of Fabricius, caecal tonsil, kidneys, liver, spleen and thymus were collected in 10 per cent formalin in sterile container for histopathological examination. Gross pathological lesions were observed in liver from 3rd dpi to 21st dpi in both the groups. Enlarged and congested kidneys were seen at 5th dpi and 7th dpi in I/M and oral group respectively. The intensity of lesions in liver, heart and kidneys were slightly higher in I/M group than the oral group. Histopathological examination of infected tissues revealed mild to severe changes were observed in all tissues except in thymus. The presence of basophilic intranuclear inclusion bodies were found in I/M group from 7th dpi whereas 21st dpi in Oral groups.