A plant secretory sequence enhances immunogenicity of electroporated COVID-19 DNA vaccines
All the DNA constructs resulted in protein expression <i>in vitro</i> and in the induction of both antibody and CD4 and CD8T cell responses in mice. EGT significantly increased DNA constructs immunogenicity, especially for the induction of antibody response, confirming its potential in DNA vaccination. Remarkably, constructs including the plant secretory signal sequence resulted to be highly expressed and triggered higher antibody and CD4T cell responses, highlighting that the combination of this sequence and EGT can be used to boost the immunogenicity of DNA-vaccine coded proteins, ultimately helping in their design.
Authors
- Silvia Massa (ORCID: https://orcid.org/0000-0001-8429-4031)
- Claudio Pioli (ORCID: https://orcid.org/0000-0002-1360-2727)
- Emanuela Pasquali (ORCID: https://orcid.org/0000-0002-3997-0659)
- Flavia Novelli (ORCID: https://orcid.org/0000-0001-9246-3704)
- Doriana Triggiani (ORCID: https://orcid.org/0000-0003-2326-1214)
- Rosella Franconi (ORCID: https://orcid.org/0000-0003-4982-1646)
- Olivia Costantina Demurtas (ORCID: https://orcid.org/0000-0003-3344-5754)
- Caterina Merla (ORCID: https://orcid.org/0000-0001-8612-9566)
Institutions
- National Agency for New Technologies Energy and Sustainable Economic Development (GB)
Publication Details
- Journal
- Frontiers in Medical Technology
- Published
- 2025-07-14
- DOI
- https://doi.org/10.3389/fmedt.2025.1597179
- Primary Topic
- Transgenic Plants and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00