Study of Collagen-Induced Platelet Aggregation Using the Method of Low Angle Light Scattering

Assessment of platelet function is of key importance for the diagnosis of bleeding disorders and monitoring the effectiveness of antiplatelet drugs. Relatively recently, a method for assessing platelet aggregation based on the phenomenon of low angle light scattering was developed. Measurements are carried out in a medium with a known pH value and calcium concentration. The aim of this work was to investigate the influence of preanalytical and analytical variables, as well as to compare the results with the light transmission aggregometry method already widely used in clinical practice. It was found that transporting the sample by car for two hours does not affect the parameters of collagen-induced aggregation. It was discovered that the low angle light scattering method for measuring collagen-induced platelet aggregation exhibits good precision under repeatable conditions and is short-term reproducible. The aggregation parameters obtained by the low angle light scattering method directly correlate with the aggregation parameters measured by the transmission aggregometry method. The accelerating role of adenosine diphosphate secreted from dense granules in collagen-induced platelet aggregation was demonstrated.