Electrosynthesis of PolyFilm Modified Graphite Electrode and its Application Towards Determination of Thymine

This article presents an electrochemical sensor designed for the identification of thymine, one of the nitrogenous bases in deoxyribonucleic acid, crucial for diagnosing damage and health issues such as cancer. The sensor was fabricated using a graphite electrode modified by electrodeposition of brilliant cresyl blue dye in a phosphate-buffered solution, employing cyclic voltammetry. The modified electrode was characterized by atomic force microscopy, revealing changes in surface morphology that facilitated the detection of thymine with a limit of 0.25 µM, demonstrating enhanced oxidation currents. The sensor’s selectivity was tested in the presence of interferents. The use of graphite-based electrochemical sensors for thymine detection has gained attention due to their sensitivity, low cost, ease of fabrication and disposal, and the growing demand for innovative technologies in medical and technological fields.