Whole genome sequence of a virulent and multidrug resistant Staphylococcus aureus strain MD02 isolated from a diabetic foot ulcer in Uganda

Genomic DNA of S. aureus strain MD02 was extracted and sequenced using the Illumina platform, generating high-quality paired-end reads with an average genome coverage of 32.6×. Quality control was performed with FastQC and reads were trimmed using Trimmomatic. De novo assembly was carried out using SPAdes, resulting in a draft genome of 2,815,980 bp assembled into 68 contigs with a GC content of 32.5%. Quality analysis of the genome revealed 98% completeness, and 2.48% contamination and the closest type strain to our isolate was Staphylococcus aureus (GCA_000330825.2) with an average nucleotide identity of 97.14%% and genome coverage of 89.38% confirming species level identity and close relatedness. Annotation using NCBI Prokaryotic Genome Annotation Pipeline identified 2,701 protein coding genes.

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Publication Details

Journal
BMC Genomic Data
Published
2025-07-12
DOI
https://doi.org/10.1186/s12863-025-01342-1
Citations
1
Primary Topic
Antimicrobial Resistance in Staphylococcus
Type
article
Field-Weighted Citation Impact
1.40
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article

Whole genome sequence of a virulent and multidrug resistant Staphylococcus aureus strain MD02 isolated from a diabetic foot ulcer in Uganda

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1 citations
BMC Genomic Data
Antimicrobial Resistance in Staphylococcus
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article

Whole genome sequence of a virulent and multidrug resistant Staphylococcus aureus strain MD02 isolated from a diabetic foot ulcer in Uganda

Ezera Agwu, Theophilus Pius, Emmanuel Eilu, Reagan Muhwezi, Saheed Adekunle Akinola, Ismail Abiola Adebayo, Martin Odoki, Murtala Bello Abubakar, Danladi Makeri, Reuben S. Maghembe, Priscilla Peter Dilli
article en
1 citations

Abstract

Genomic DNA of S. aureus strain MD02 was extracted and sequenced using the Illumina platform, generating high-quality paired-end reads with an average genome coverage of 32.6×. Quality control was performed with FastQC and reads were trimmed using Trimmomatic. De novo assembly was carried out using SPAdes, resulting in a draft genome of 2,815,980 bp assembled into 68 contigs with a GC content of 32.5%. Quality analysis of the genome revealed 98% completeness, and 2.48% contamination and the closest type strain to our isolate was Staphylococcus aureus (GCA_000330825.2) with an average nucleotide identity of 97.14%% and genome coverage of 89.38% confirming species level identity and close relatedness. Annotation using NCBI Prokaryotic Genome Annotation Pipeline identified 2,701 protein coding genes.

BMC Genomic DataVol. 26(1)
Nkumba University (UG), Kampala International University (UG), St. Augustine International University (UG), Kabale University (UG), University of Rwanda (RW), Makerere University (UG)
Zero hunger
Openalex Percentile: Top 18%
Antimicrobial Resistance in Staphylococcus
1.40
DOIOpen Access PDFOpenAlex
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