<b><i>Pasteurella multocida</i> genome assembly of Indonesian local isolates using long-read Oxford Nanopore Technology sequencing</b>

This study successfully assembled the genomes of two <i>P. multocida</i> isolates from Lampung (2,952) and Kupang, Nusa Tenggara Timur, Indonesia, using Oxford Nanopore Technologies' long-read sequencing. The resulting single-contig genomes, measuring 2.35 and 2.29 Mbp, respectively, exhibited high completeness scores (BUSCO: >99%). These high-quality assemblies enhance genomic contiguity, providing valuable resources for functional analyses aimed at understanding the virulence factors and pathogenesis of HS in Indonesian livestock.

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Publication Details

Journal
Open Veterinary Journal
Published
2025-01-01
DOI
https://doi.org/10.5455/ovj.2025.v15.i6.46
Primary Topic
Microbial infections and disease research
Type
article
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article

Pasteurella multocida genome assembly of Indonesian local isolates using long-read Oxford Nanopore Technology sequencing

Susan Maphilindawati Noor, I Wayan Teguh Wibawan, Heri Kurnianto, Safika Safika et al.
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article

Pasteurella multocida genome assembly of Indonesian local isolates using long-read Oxford Nanopore Technology sequencing

Susan Maphilindawati Noor, I Wayan Teguh Wibawan, Heri Kurnianto, Safika Safika, Susanti Susanti, Sumarningsih Sumarningsih, Harimurti Nuradji, Aswin Rafif Khairullah, Sri Suryatmiati Prihandani, Yudi Adinata, Sutiastuti Wahyuwardani, Tati Ariyanti, Andriani Andriani, Alif Rahman Rohim Puarada
article en

Abstract

This study successfully assembled the genomes of two <i>P. multocida</i> isolates from Lampung (2,952) and Kupang, Nusa Tenggara Timur, Indonesia, using Oxford Nanopore Technologies' long-read sequencing. The resulting single-contig genomes, measuring 2.35 and 2.29 Mbp, respectively, exhibited high completeness scores (BUSCO: >99%). These high-quality assemblies enhance genomic contiguity, providing valuable resources for functional analyses aimed at understanding the virulence factors and pathogenesis of HS in Indonesian livestock.

Open Veterinary JournalVol. 15(6)
Indonesian Agency for Agricultural Research and Development (ID), National Research and Innovation Agency (ID)
Openalex Percentile: Top 84%
Microbial infections and disease research
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