Four Ab Initio Whole Cell Kinetic Models of Bacillus subtilis 168 (bsuLL25) 6051-HGW (bshSM25), N33 (bsuN33SS25), FUA2231 (bsuGR25)

Bacillus subtilis has been used in synthetic biology and metabolic engineering due to its generally recognized as safe status, its tolerance to harsh conditions and high protein expression and secretion abilities. Whole cell kinetic models are useful for in silico screening and evaluation of engineering approaches prior to experimental manipulations. However, there is no whole cell KM of B. subtilis to date. In this study, we present four whole cell KMs of B. subtilis – one KM for each strain of B. subtilis; namely, 168, 6051-HGW, N33 and FUA2231; as models bsuLL25, bshSM25, bsuN33SS25 and bsuGR25 respectively. Each of these four KMs contain between 1300 and 1565 reactions, catalyzes by between 571 and 679 enzyme types; which uses between 1053 and 1681 metabolites. These models can be a baseline models for incorporating other cellular and growth processes or as a system to examine cellular resource allocations necessary for engineering.