Assessment of the ability of Bacillus velezensis bacteria to produce cyclic lipopeptides and characteristics of their growth-stimulating and bioremediation properties

In recent years, agriculture has faced the problem of finding promising drugs that are effective in combating plant diseases, alternatives to synthetic pesticides that can have a negative impact on both living organisms and the environment as a whole. To solve this problem, the possibility of using biological methods of protecting agricultural crops is being studied. In particular, special attention is paid to the search for new strains of bacteria – natural antagonists of pathogenic microorganisms that could be used instead of synthetic chemicals. The aim of this study was to assess the ability of Bacillus velezensis HR13 bacteria to produce secondary metabolites with antagonistic and growth-stimulating properties. The cyclic lipopeptides identified in the bacterial culture fluid were characterized using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS). The positive effect of B. velezensis HR13 bacteria on the morphometric parameters of plant seedlings was shown when inoculated with the studied strain at a concentration of 106 – 107 m.c./ml. The ability of B. velezensis HR13 bacteria to use representatives of such groups of active substances of synthetic fungicides as triazoles and phenylpyrroles in various concentrations as the sole carbon source was determined.