Express determination of ceftriaxone using immobilized copper sulfate (II)

Indicator papers based on immobilized copper (II) sulfate have been obtained for the determination of theβ-lactam antibiotic ceftriaxone. The interaction of immobilized copper (II) sulfate with ceftriaxone has been assessed using diffuse reflectance spectroscopy. Methods have been developed for visual colorimetric and colorimetric determination of the antibiotic using a smartphone camera, Adobe Photoshop CS5® software, and a regression model built using the Python programming language. Color test scales for rapid assessment of ceftriaxone content have been obtained. Metrological characteristics of visual colorimetric determination of the antibiotic have been established: the range of determined contents is 0,50–64 mg/ml, the unreliability interval is 0,07–0,40 mg/ml, the detection limit is 0,40 mg/ml. For colorimetric assessment of ceftriaxone concentration, linear dependencies of the intensities of the color parameters RGBHSVCMYK on the decimal logarithm of the antibiotic concentration have been constructed. The profiles of petal diagrams in the coordinates of color parameters RGBHSV were obtained and the dependences of the parameters of areas and perimeters on the decimal logarithm of the concentration of the antibiotic have been established. The range of determined contents in colorimetric determination was 0,10–64 mg/ml. A decrease in the value of the detection limit in the colorimetric determination of ceftriaxone has been noted (DL = 0,05). An assessment of the correctness of the express-determination of ceftriaxone by the “introduced–found” method has been carried out, Sr does not exceed 0,08.