CYTOTOXIC ACTIVITY OF XANTHONES FROM THE TWIGS AND LEAVES OF CRATOXYLUM COCHINCHINENSIS (LOUR.) BLUME

This study aimed to isolate and identify bioactive compounds from the ethyl acetate extract of Cratoxylum cochinchinensis twigs and leaves. As a result, five xanthones were obtained and structurally characterized as 1,8-dihydroxy-3-methoxy-6-methylxanthone (1), cochinchinone A (2), 1,3,7-trihydroxy-2,4-diisoprenylxanthone (3), α-mangostin (4), and 1,3,5,6-tetrahydroxy-7-isoprenylxanthone (5). Notably, compounds 1 and 5 were isolated for the first time from C. cochinchinensis. Furthermore, the cytotoxic activity of these compounds was evaluated in vitro against three cancer cell lines (A549, MCF-7, and HepG2). Among them, compounds 2-4 exhibited cytotoxic activities against all tested cell lines, with IC50 values ranging from 10.24 to 16.86 µM. Meanwhile, compound 5 showed selective activity against MCF-7 and HepG2, with IC50 values of 13.55 ± 0.74 and 16.69 ± 0.81 µM, respectively.