Design develop and optimize a novel functional excipient fulvic acid appended topical nano emulsion containing thymoquinone for prolonged remission of psoriasis

The aim and objective of the proposed work, was to develop Fulvic acid and thymoquinone loaded nanoemulsion that could deliver the drug topically at the affected site of the diseased skin and to test the efficacy and safety of the formulated nanoemulsion through in-vitro and in-vivo study. Molecular docking studies showed the docking scores of thymoquinone and fulvic acid were -4.4 and -6.0 kcal/mol with interleukin-6 and the residues Leu11, Val118, Thr93, Ala175, Tyr183 form polar hydrogen bond with the ligands as well as hydrophobic bond interaction. 6.1, 6.2 and 6.3). After molecular docking study by Auto dock vina tools the results indicated that the fulvic acid have significant binding score (-6.0 and 6.4 kcal/mol) and high affinity with both receptors. Conclusively, the purported work explores a fulvic acid based thymoquinone nanoemulsion gel/ nanoemulgel using ultrasonic technique and "Quality by Design" (QbD) approaches. The concept of nanoemulgel was adopted to enhance solubility, absorption and bioavailability of a thymoquinone for topical as well as locoregional application. NE‘s size has resulted in combination therapy of TMQ and FA which utilised kalonji as the oil phase and Tween 80 as the surfactant, resulting as a perfect carrier for inclusion of poorly water-soluble medications (TMQ, FA), and enhanced penetration through the skin.