The proton-dependent oligopeptide transporter Ptr_C serves as an efficient system for antifungal delivery into <i>Candida auris</i>
In this study, utilizing deletion mutants of C. auris PTR transporters, we demonstrate that Ptr_C is the primary transporter facilitating the uptake of FMDP-based antifungal peptides. Furthermore, we developed a Ptr_C overexpression system in Saccharomyces cerevisiae, enabling rapid identification of antifungal peptides capable of exploiting this transporter using simplistic growth-based assays. The system's competence was further validated by constructing a few key site-directed mutants that alter the functional specificity of Ptr_C.
Authors
- Amresh Prakash (ORCID: https://orcid.org/0000-0002-4821-1654)
- Ryszard Andruszkiewicz (ORCID: https://orcid.org/0000-0002-6144-2078)
- Sławomir Milewski (ORCID: https://orcid.org/0000-0003-2616-4495)
- Atanu Banerjee (ORCID: https://orcid.org/0000-0001-9319-4593)
- Sheheryar Malik (ORCID: https://orcid.org/0000-0001-7411-0729)
- Andrzej S. Skwarecki (ORCID: https://orcid.org/0000-0002-7070-6071)
- Rosy Khatoon
- Rajendra Prasad (ORCID: https://orcid.org/0000-0001-6061-001X)
- Amandeep Saini (ORCID: https://orcid.org/0000-0001-8216-5439)
Institutions
- Gdańsk University of Technology (PL)
- University of Gdańsk (PL)
- Amity University (AE)
Publication Details
- Journal
- Journal of Applied Microbiology
- Published
- 2025-07-01
- DOI
- https://doi.org/10.1093/jambio/lxaf170
- Primary Topic
- Antifungal resistance and susceptibility
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- European Molecular Biology Organization
- Department of Biotechnology, Government of West Bengal
- Science and Engineering Research Board