Simultaneous Estimation of Amoxicillin Trihydrate, Cloxacillin Sodium and Probenecid in Tablet Formulation by UV-Spectrophotometric Method

Amoxycillin trihydrate, cloxacillin trihydrate and probencid are proven for their therapeutic benefits to treat bacterial infection. There are numerous approaches available for the simultaneous estimation of two medicines, but there is none accessible for the estimation of three medications, or the ones that exist are quite complicated, so the current study focuses on developing and validates a new, simple, rapid and novel spectrophotometric method for simultaneously estimating amoxicillin trihydrate, cloxacillin sodium, and probenecid in pure and solid dose forms using the simultaneous equation method. The λmax of Amoxicillin Trihydrate (AMOX) (10 μg/ml), Cloxacillin Sodium (CLOX) (10 μg/ml), and Probenecid (PROB) (10 μg/ml) were 279.89 nm, 219.02 nm, and 239.67 nm, respectively. The medications followed Beer's rule and showed a good connection. Calibration curves for AMOX, CLOX, and PROB were linear from 5 to 30 μg/ml. The results indicated that the technique was accurate, precise, and reproducible (with a relative standard deviation of less than 1%) as per ICH guidelines. It is also affordable, simple to use, and time-saving, making it appropriate for the simultaneous determination of three medicines in commercial preparations. The proposed methods are recommended for routine analysis since they are rapid, simple, accurate, cost effective and also sensitive and specific. It involves neither heating nor use of any organic solvent for separation of the combination. Thus, this study exploits the possibility for determining Pharmacokinetic data of the combined formulation which may be required in pre-clinical and clinical studies in near future.