Bacteriological Profile of Pus Samples and Their Antibiotic Susceptibility Pattern at A Tertiary Care Hospital, Jamnagar, Gujarat, India'

Background: Pyogenic infections are a significant clinical concern, often caused by a variety of multidrugresistant organisms, particularly in hospitalized patients.The increasing trend of resistance in P. aeruginosa and other Gram-negative organisms highlights the need for updated local susceptibility data to guide empirical therapy. Methods: This retrospective observational study was conducted from September 2024 to February 2025 at M.P. Shah Government Medical College, Jamnagar. A total of 1000 pus samples were analyzed, of which 322 (32.2%) showed positive bacterial growth. The isolates were identified by standard microbiological methods and antibiotic susceptibility testing was performed using the Kirby-Bauer disk diffusion method, interpreted according to CLSI guidelines. Results: Out of 322 culture-positive isolates, Gram-negative bacteria were predominant (52.5%), with P. aeruginosa being the most frequently isolated organism (35.4%), followed by E. coli (16.1%) and Proteus spp. (12.7%). Among Gram-positive organisms, Staphylococcus aureus was most common (12.7%), with nearly half (48.1%) identified as MRSA. Carbapenems (meropenem, imipenem) showed high sensitivity against Gramnegative isolates, while vancomycin and linezolid were most effective against Gram-positive isolates. ESBL production was observed in 61.2% of Gram-negative isolates. Conclusion: The study emphasizes a high burden of multidrug-resistant pathogens in wound infections, particularly among Gram-negative organisms. Continued surveillance and strict antimicrobial stewardship are crucial to mitigate resistance. Carbapenems and beta-lactam/beta-lactamase inhibitor combinations remain the most effective empirical options. The findings support the need for hospital-specific antibiotic policies and infection control strategies