Protein Isolation and Purification of Sea Cucumber (Holothuria sp. and Stichopus sp.) from Sepanjang Beach, Yogyakarta

Holothuria sp. and Stichopus sp. at Sepanjang Beach, Yogyakarta were studied for protein isolation. The crude protein concentrations in the body wall and intestine of Holothuria sp. were 2.42% and 2.13%, while Stichopus sp. had 4.03% and 4.01%, respectively. Proteins identified through SDS-PAGE analysis included collagen, softenin, and actin. Purified protein concentrations in Holothuria sp. were 3.72 ± 0.04 μg mL⁻¹ (body wall) and 4.09 ± 0.05 μg mL⁻¹ (intestine), while Stichopus sp. had 3.98 ± 0.08 μg mL⁻¹ and 9.34 ± 0.48 μg mL⁻¹, respectively. This study supports nutraceutical development, identifying potential health-enhancing supplements and drugs.