Klotho gene transfection into mesenchymal stem cells as a potential therapy for kidney disease

Purpose: Chronic kidney disease (CKD) is a progressive condition associated with high morbidity and mortality. Traditional therapeutic strategies primarily aim to manage symptoms and slow disease progression, but often fail to reverse kidney damage. The mesenchymal stem cells (MSCs), known for their regenerative and immunomodulatory capacities, have emerged as promising candidates for renal repair. Meanwhile, the Klotho gene, recognized as an anti-aging and nephroprotective gene, is markedly downregulated in CKD. This review conducted the synergistic potential of combining MSC therapy with Klotho gene transfection. By enhancing MSCs with Klotho, their reparative effects, such as anti-fibrotic, anti-inflammatory, and antioxidant properties, can be boosted. Materials and Methods: the search process covered over 50 peer-reviewed articles and research papers, about the Mesenchymal stem cell therapy in kidney repair disease with different transfection protocols, and the same as the klotho gene, with an understanding of the therapeutic role. However, all databases in this review were estimated by PubMed and Google Scholar with keywords. Conclude: the biology of the Klotho gene, MSC-based therapeutic mechanisms, current transfection techniques, preclinical studies, and translational challenges. This approach may offer a novel and more effective therapeutic avenue for kidney disease management.