Genomic islands and mobilome demonstrate a potential pathogen, the Plesiomonas shigelloides, as in Nile tilapia (Oreochromis niloticus)

Plesiomonas shigelloides is a common opportunistic pathogen of fish that may result in significant economic losses during outbreaks periods. The infection is revealed by several clinical signs, including pericarditis, multifocal necrosis, hepatic atrophy, ulcerative lesions and other pathological changes, which may result in a reduction in the number of economically viable animals on fish farms. In humans, P. shigelloides infections are associated with diarrhoeic cases resulting from the consumption of contaminated food. These facts justify further study of the species pathogenicity, which is not yet well-established owing to the recent taxonomic classification. Considering the aforementioned information, the main goal of this research was to characterize the virulence and antimicrobial resistance factors present in four P. shigelloides strains isolated from aquatic organisms in Brazil. To gain a deeper understanding of the genomic characteristics of the subjects, the following methodology was employed: the genomic similarity of the strains was evaluated through the pyANI method; the phylogenomic evaluation was performed by OrthoFinder; GIPSy software was used for genomic island searches; and PanViTa was employed to search for virulence and resistance factors. The results suggest the presence of multiple virulence factors (51) and antimicrobial resistance genes (12) in the genomes of the main isolates. This is the first genomic study of P. shigelloides exploring the potential pathogenicity in Nile tilapia in the northeast Brazil. • Elucidate and evaluate the virulome profile of new strains of P. shigelloides • Genomic data associated to epidemic potential of P. shigelloides • Understand the pathogenicity of Plesiomonas and its global distribution • P. shigelloides is an emerging pathogen that can cause great economic losses

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Publication Details

Journal
The Microbe
Published
2025-07-05
DOI
https://doi.org/10.1016/j.microb.2025.100441
Citations
1
Primary Topic
Aquaculture disease management and microbiota
Type
article
Field-Weighted Citation Impact
0.80
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article

Genomic islands and mobilome demonstrate a potential pathogen, the Plesiomonas shigelloides, as in Nile tilapia (Oreochromis niloticus)

Rodrigo Dias de Oliveira Carvalho, Andrew Macrae, Flávia Figueira Aburjaile, Raquel Hurtado et al.
1 citations
The Microbe
Aquaculture disease management and microbiota
0.80
article

Genomic islands and mobilome demonstrate a potential pathogen, the Plesiomonas shigelloides, as in Nile tilapia (Oreochromis niloticus)

Rodrigo Dias de Oliveira Carvalho, Andrew Macrae, Flávia Figueira Aburjaile, Raquel Hurtado, João Ricardo Vidal Amaral, Sílvia De Carli, Thiago J. Sousa, Siomar de Castro Soares, Mateus Matiuzzi da Costa, Bertram Brenig, Franciele Maboni Siqueira, Gisele Veneroni Gouveia, Sheila da Silva, Lígia Carolina da Silva Prado, Diego Lucas Neres Rodrigues, João José de Simoni Gouveia, Ulisses de Pádua Pereira, Selma Soares de Oliveira, Rommel Thiago Jucá Ramos, Juan Carlos Ariute, M. S. T. Batista, Aristóteles Goés‐Neto, Vasco Azevedo, Elinaldo Bernardo de Oliveira Júnior
article en
1 citations

Abstract

Plesiomonas shigelloides is a common opportunistic pathogen of fish that may result in significant economic losses during outbreaks periods. The infection is revealed by several clinical signs, including pericarditis, multifocal necrosis, hepatic atrophy, ulcerative lesions and other pathological changes, which may result in a reduction in the number of economically viable animals on fish farms. In humans, P. shigelloides infections are associated with diarrhoeic cases resulting from the consumption of contaminated food. These facts justify further study of the species pathogenicity, which is not yet well-established owing to the recent taxonomic classification. Considering the aforementioned information, the main goal of this research was to characterize the virulence and antimicrobial resistance factors present in four P. shigelloides strains isolated from aquatic organisms in Brazil. To gain a deeper understanding of the genomic characteristics of the subjects, the following methodology was employed: the genomic similarity of the strains was evaluated through the pyANI method; the phylogenomic evaluation was performed by OrthoFinder; GIPSy software was used for genomic island searches; and PanViTa was employed to search for virulence and resistance factors. The results suggest the presence of multiple virulence factors (51) and antimicrobial resistance genes (12) in the genomes of the main isolates. This is the first genomic study of P. shigelloides exploring the potential pathogenicity in Nile tilapia in the northeast Brazil. • Elucidate and evaluate the virulome profile of new strains of P. shigelloides • Genomic data associated to epidemic potential of P. shigelloides • Understand the pathogenicity of Plesiomonas and its global distribution • P. shigelloides is an emerging pathogen that can cause great economic losses

The MicrobeVol. 8
Universidade Federal de Minas Gerais (BR), Universidade Federal do Rio de Janeiro (BR), Universidade Federal do Rio Grande do Sul (BR), Universidade Federal do Triângulo Mineiro (BR), Universidade Federal do Vale do São Francisco (BR), Universidade Federal do Pará (BR), University of Göttingen (DE)
Life below water
Openalex Percentile: Top 26%
Aquaculture disease management and microbiota
0.80
DOIOpen Access PDFOpenAlex
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