Antinociceptive and Anti‐inflammatory Effect of Nicandrin B Isolated From <i>Datura ferox</i> in Zebrafish
Nicandrin B (Nic B), a withanolide isolated from Datura ferox leaves, was investigated for its antinociceptive and anti-inflammatory effects in adult zebrafish (Danio rerio). Animals treated with Nic B (4, 20, and 40 mg/kg) showed no toxicity and maintained normal locomotor activity. The compound significantly reduced nociception induced by formalin and hypertonic saline; these effects were reversed by TRPA1 and transient receptor potential vanilloid 1 antagonists, indicating neuromodulation of these targets. Additionally, Nic B attenuated carrageenan-induced abdominal edema, reduced neutrophil recruitment, and decreased hepatic reactive oxygen species levels. Docking analysis confirmed a favorable binding affinity with TRPA1, supporting its therapeutic potential. These findings suggest that Nic B combines analgesic, anti-inflammatory activity, and toxicological safety, making it a promising drug candidate.
Authors
- Matheus Nunes da Rocha (ORCID: https://orcid.org/0000-0002-0929-4565)
- Jéssica Bezerra Maciel (ORCID: https://orcid.org/0000-0002-8626-1257)
- Otília Deusdênia Loiola Pessoa (ORCID: https://orcid.org/0000-0002-1617-7586)
- Maria Kueirislene Amâncio Ferreira (ORCID: https://orcid.org/0000-0002-4270-8109)
- Hélcio Silva dos Santos (ORCID: https://orcid.org/0000-0001-5527-164X)
- Antônio Wlisses da Silva (ORCID: https://orcid.org/0000-0002-1686-1644)
- Emanuela de Lima Rebouças (ORCID: https://orcid.org/0000-0002-0572-5196)
- Francisco das Chagas Lima Pinto (ORCID: https://orcid.org/0000-0003-1481-7970)
- Jane Eire Silva Alencar de Menezes (ORCID: https://orcid.org/0000-0001-9632-5464)
- Emmanuel Silva Marinho (ORCID: https://orcid.org/0000-0002-4774-8775)
- Janaína Serra Azul Monteiro Evangelista (ORCID: https://orcid.org/0000-0002-9583-1573)
- Andreia Ferreira de Castro Gomes (ORCID: https://orcid.org/0000-0002-0567-064X)
- Márcia Machado Marinho (ORCID: https://orcid.org/0000-0002-7640-2220)
- Marília Pereira (ORCID: https://orcid.org/0000-0001-7347-3480)
- Izabel Florindo Guedes
Institutions
- Universidade Estadual do Ceará (BR)
- Universidade Federal do Ceará (BR)
- State University of Vale do Acaraú (BR)
- University of Minho (PT)
Publication Details
- Journal
- Chemistry & Biodiversity
- Published
- 2025-07-05
- DOI
- https://doi.org/10.1002/cbdv.202501221
- Primary Topic
- Phytochemicals and Medicinal Plants
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico
- Universidade Estadual do Ceará