Pinus monophylla (Pinaceae) resin: A novel technique for extracting terpe-noids from aromatic plant materials

Pinus monophylla is an aromatic tree in the Pinaceae family, that grows throughout the western United States.This conifer species naturally exudes large quantities of essential oil-rich resins, which, similar to other commercially available tree resins, can be hydrodistilled to extract the essential oil (EO).Following an initial hydrodistillation, a patents pending process was used herein, in which the resin EO was used as a solvent to extract additional compounds from the spent conifer resin, including non-volatile terpenoids.GC/MS and LC/MS analyses of samples (n = 6) were conducted to establish the terpenoid profiles of both EO and secondary extracted samples (DeepSpectra samples).The volatile profiles of P. monophylla resin extractions were somewhat similar between sample types, EO and DeepSpectra samples, with prominent compounds in pinene (avg.83.2%, 80.0%), -3-carene (avg.2.4%, 2.2%), and -copaene (avg.2.7%, 3.5%), respectively.DeepSpectra samples contained abietic acid (avg.6.3 mg/mL) as well as other non-volatile compounds, although they were not detected in the EO samples.To the best of our knowledge, this is the first study to establish the complete terpenoid profile of P. monophylla resin using a novel sequential extraction technique.