Structure‐Guided Engineering of Thermodynamically Enhanced SaCas9 for Improved Gene Suppression (Adv. Mater. 26/2025)
Thermostable Protein Engineering In article number 2404680, Trung Thanh Thach, Jaekyung Hyun Yong Ho Kim, and co-workers introduce a structure-guided engineered SaCas9 variant with enhanced thermostability and compactness, enabling efficient gene suppression through nonviral delivery. The engineered sdCas9-KRAB-R system demonstrates superior nuclear localization and in vivo knockdown efficiency, showcasing its potential as a robust tool for CRISPR interference and targeted genomic regulation.
Authors
- H.‐S. KIM
- Kilho Eom (ORCID: https://orcid.org/0000-0001-5135-8473)
- Jaekyung Hyun (ORCID: https://orcid.org/0000-0003-2914-537X)
- Bok‐Soo Lee
- Trung Thanh Thach (ORCID: https://orcid.org/0000-0001-8395-0623)
- Young Hyun No (ORCID: https://orcid.org/0000-0002-3653-6339)
- Zinah Hilal Khaleel (ORCID: https://orcid.org/0000-0003-4358-9516)
- Eun Sung Kang (ORCID: https://orcid.org/0000-0002-5090-9875)
- Hyeyeon Jeon (ORCID: https://orcid.org/0009-0007-7387-5667)
- Hyun‐Kyoung Lim (ORCID: https://orcid.org/0000-0003-1818-420X)
- Jiyoung Nam (ORCID: https://orcid.org/0000-0002-1351-2262)
- Minah Suh (ORCID: https://orcid.org/0000-0002-1393-3550)
- Nam Hyeong Kim (ORCID: https://orcid.org/0000-0001-8489-5152)
- Kayoung Han (ORCID: https://orcid.org/0000-0003-3911-4104)
- Kyung‐Tae Kim (ORCID: https://orcid.org/0009-0001-8562-4992)
- Yong Ho Kim (ORCID: https://orcid.org/0000-0002-9106-3298)
- Jaecheol Lee (ORCID: https://orcid.org/0000-0002-2070-7463)
- Dongsun Shin (ORCID: https://orcid.org/0009-0006-3175-3293)
Institutions
- Suwon Research Institute (KR)
- Institute for Basic Science (KR)
- Sungkyunkwan University (KR)
Publication Details
- Journal
- Advanced Materials
- Published
- 2025-07-01
- DOI
- https://doi.org/10.1002/adma.202570177
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00