Ligation-induced DNA self-assembly
Ligation is a common treatment for the resulting DNA nanostructures to gain extra stability. In this study, we aim to utilize ligation as an active process in self-assembly instead of post-assembly stabilization. Our investigation focuses on constructs with transiently paired segments. Transient base pairing fails to hold up the assembled complex, but ligation treatment turns the transient base pairing into permanent ones and thus induces the desired self-assembly. We apply the method to a number of assembly tasks, leading to the successful construction of discrete and extended DNA nanostructures. Furthermore, we apply the ligation-based method in the hierarchical assembly of preformed DNA nanostructure units into higher-order superstructures.
Authors
- Wen Wang (ORCID: https://orcid.org/0000-0003-3359-1030)
- Xue Lei (ORCID: https://orcid.org/0000-0001-9647-3782)
- Bryan Wei (ORCID: https://orcid.org/0000-0003-2515-2409)
- Qishu Zhang (ORCID: https://orcid.org/0000-0002-2982-1537)
- Yue Shen (ORCID: https://orcid.org/0000-0002-3276-7295)
- Yao Wang (ORCID: https://orcid.org/0000-0002-3440-1801)
- Jianqiu Zhao
- Zhengyang Sun
Institutions
- Botswana Geoscience Institute (BW)
- BGI Research (CN)
- Tsinghua University (CN)
Publication Details
- Journal
- Nucleic Acids Research
- Published
- 2025-06-20
- DOI
- https://doi.org/10.1093/nar/gkaf570
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Ministry of Science and Technology of the People's Republic of China
- Tsinghua University
- Jiangsu Science and Technology Department
- National Key Research and Development Program of China