Calotropis Procera Extract Ameliorated Thyroid Toxicity Of Magnetic Iron Oxide Nanoparticles In Rats Viatlr4 /MYD88/NF-Κb Inflammatory Pathway

Background: Magnetic iron oxide nanoparticles (MIONPs) are widely used in many fields of life including the medical field, food and textile industries due to their unique properties. The long-term effects of MIONPs on the body are not fully studied. In addition natural products have emerged in the medical field as protective agents against toxic substances. Objective: The purpose of our investigation was to determine the effectiveness of Calotropis procera (CP) extract on MIONPs induced thyroid function alternation in a rat model. Methods: The study was conducted on eighty male Wistar rats (100-150g). Rats were allocated randomly into 4 groups of 20 rats; group (1) control (only tape water), group (2) CP extract only per oral (500 mg/kg Bw) daily, group (3) MIONPs only per oral (50 mg/kg Bw) daily and group (4) MIONPs (50 mg/kg Bw) plus CP extract per oral (500 mg/kg Bw) daily. At the end of the 30th day, Animals were sedated, and samples were obtained right away for assessment of tissue levels of MDA and GPX, Quantitative analysis of NF-κB, Western blotting with Protein-tec Kits, and serum hormone evaluation in addition to evaluation of apoptosis in thyroid tissue. Data were collected, tabulated and statistically analyzed. Results: MIONPs intoxication caused a non-significant decrease in serum T3, T4 and tissue GXP. Also significant increase resulted in serumNF-κB, TLR4 and MYD88 proteins expression and tissue MDA level, while non-significant increase was noticed in serum TSH and BAX gene expression.After treatment with CP extract, there was non-significant increase in serum T4 but non-significant decrease was in serum TSH and BAX gene expression. TLR4 /MYD88/NF-κB axis showed significant decrease after treatment with CP. No conclusive results were noticed from Bcl2 measurement. Conclusions: MIONPs induced a toxic effect on thyroid gland through increased the levels of thyroid tissue MDA, NF-κB in addition to the protein expression of TLR4 and MYD 88. CP extract could ameliorate the MIONPs induced thyroid toxicity viaTLR4 /MYD88/NF-κB inflammatory pathway.