Molecular mechanisms of skin biorevitalization: expression of signaling molecules under the action of trehalose-containing gel

Introduction: Biorevitalization is a modern method of injection cosmetology aimed at correcting age-related skin changes by introducing hyaluronic acid (HA) and additional biologically active components. The hyaluronic gel for microimplantation and mesotherapy, containing trehalose, differs from classical biorevitalizants by its complex action, aimed not only at moisturizing the skin, but also at stimulating its regenerative processes. Purpose of the study: The purpose of this work was to study the features of the molecular mechanisms of skin biorevitalization under the action of the trehalose-containing gel. Material and methods: Using the immunohistochemistry (IHC) method, which provides high selectivity in the detection and localization of molecules with antigenic properties in tissues, the expression of Aquaporin 3, NF-κB, PINK1, Parkin biomarkers in skin biopsies of patients before and after treatment was determined. Results: The combination of hyaluronic acid and trehalose demonstrated a complex effect of the drug with the manifestation of molecular biological effects: skin hydration without the risk of hyperhydration, stimulation of mitophagy and metabolic activity, moderate regulation of inflammation, which generally contributes to physiological effective biorevitalization. Conclusion. Injections of a gel based on hyaluronic acid and trehalose help to reduce dense collagen type I and the expression of Aquaporin 3, which increases the hydrophilicity of the dermal matrix and mitochondrial metabolic activity. Histological and immunohistochemical data confirmed that the combination of such components activates regeneration, improves skin hydration and reduces inflammation, which contributes to successful biorevitalization.