In Vitro Study of Tryptanthrin and its Derivatives: A Potent Alkaloid Compound from Fluorescent Bacteria Pseudomonas sps in Anticancer Effects through Drug Design

ABSTRACT: Tryptanthrin, a naturally occurring indoloquinazoline alkaloid, exhibits remarkable anticancer potential. This study explores the isolation of Pseudomonas spp. from soil as a novel microbial source of tryptanthrin and its derivatives, followed by comprehensive in vitro and in silico analyses to evaluate their anticancer activity. Soil samples were collected and processed through serial dilution to isolate fluorescent Pseudomonas species, which were then identified using morphological, biochemical, and molecular (DNA extraction and sequencing) techniques. Tryptanthrin was extracted from the bacterial culture and further synthesized chemically to improve yield and structural diversity.Eight derivatives of tryptanthrin were identified through GC-MS and LC-MS analyses, with prior separation conducted via thin-layer chromatography (TLC). The anticancer potential of these compounds was investigated using molecular docking against key cancer-associated proteins, including topoisomerase II and EGFR. Protein and ligand structures were prepared and optimized for docking simulations, which revealed strong binding affinities and favorable interaction profiles, suggesting possible mechanisms of anticancer action.This integrated approach highlights the significance of microbial alkaloids, particularly from Pseudomonas spp., as a promising source of bioactive compounds. The findings support further preclinical development of tryptanthrin-based derivatives as novel anticancer therapeutics.