Correlation peculiarities between morphometric and flow cytometric indexes in the rat hippocampus and somatosensory cortex afterward cerebral ischemia-reperfusion and its correction

The increase in the ischaemic stroke incidence has become one of the main problems in the world due to high its disablement and mortality. The purpose of the study was to establish and analyse the correlations between the number of affected neuronal nuclei in the somatosensory cortex and hippocampus and flow cytometric indexes in rats having model cerebral ischaemia-reperfusion. The effects of mesenchymal stromal cells (acquired from human umbilical cord, rat and human adipose tissue, and rat embryonic fibroblasts and their lysate) on morphometric and flow cytometric parameters in the hippocampus and somatosensory cortex of adult Wistar rats (at the age – 3-4 months old, with a body weight – 160-190 g, after model cerebral ischaemia-reperfusion) were explored. The neuronal nuclei total numbers per 1 mm and the ratio of the intact neuronal nuclei number to the pathologically affected neuronal nuclei number (having karyopyknosis or karyorrhexis) were counted in the somatosensory cortex and hippocampal CA1 area. Nonparametric Spearman’s correlation rank analysis was used to determine relationships between individual parameters. When using mesenchymal stromal cells having different ancestry and their lysate as therapy for ischaemia-reperfusion damage to brain structures, multidirectional correlations (both direct and inverse) were found between flow cytometry parameters and the affected neuronal nuclei number on day 7 and 14 after the ischaemia-reperfusion modelling, both in the somatosensory cortex and in the hippocampus. Thus, the results of the correlation analysis demonstrated that mesenchymal stromal cells of different ancestry have a distinct neuroprotective effect aimed at restoring neurogenesis in brain structures and suppressing the intensity of neuroapoptosis in post-perfusion injuries. The data obtained from the correlation analysis will be used to determine the most effective stem cells’ class as a neuroprotectant and further develop an injectable drug based on it for the ischaemic stroke therapy