Extraction, Separataion, And Chemical Characterisation Of Salvadora Persica Using Gc-Ms, Nmr, And Ftir

Abstract Salvadora persica L. (Miswak/Siwak) has been used for oral hygiene for over 7,000 years, particularly in Islamic countries. This study investigates the phytochemical profile and therapeutic potential of S. persica (roots/stems), focusing on its applications for oral and microbial diseases. Successive Soxhlet extractions with petroleum ether, ethyl acetate, and ethanol were employed to isolate bioactive compounds. Functional groups and chemical constituents were characterised via FTIR, GC-MS, and NMR (1H/13C). Key findings include benzyl nitrile, dibutyl phthalate, and cis-10-heptadecenoic acid, the last of which was the most abundant. These compounds correlate with the plant’s antimicrobial, antifungal, and anti-inflammatory properties. These results underscore S. persica’s potential as a sustainable resource for biomedical applications.