News beyond our pages - July 2025

Translational studies involving adoptive transfer of tolerogenic regulatory T (Treg) cells have been hindered by phenotypic instability of in vitro-induced Treg (iTreg) cells. Chen et al (Nature 2025 Mar 26; https://doi.org/10.1038/s41586-025-08795-5) conducted an unbiased genome-wide screen using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology in primary human T cells to identify novel regulators of FOXP3 expression. Candidates were validated by integrating transcriptomic profiling and intracellular protein abundance at a single-cell level.