Antibacterial and Antioxidant Potential of Silver Nanoparticles Bio-Friendly Synthesis Using Delftia acidovorans (OM838393.1) Strain

Abstract Background: The burgeoning field of nanotechnology has witnessed a surge in the biological synthesis of nanoparticles, primarily due to their multiple applications in various domains. Objectives: This study delves into the biosynthesis of silver nanoparticles (AgNPs) utilizing the bacterium Delftia acidovorans (OM838393.1) strain and then determines their anti-MDR bacterial and antioxidant activity ( in vitro ) by DPPH assay. Materials and Methods: Bio-synthesis of AgNPs from Delftia acidovorans (OM838393.1) strain, characterized by using UV-visible spectroscopy, scanning electron microscopy (SEM), and X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR analysis) and determination their antibacterial and antioxidant activity. Results: UV-visible revealed a peak of 480 nm, crystalline nature of the biosynthesized nanoparticles with an average size of 69.7 nm scale with spherical shape. The synthesized AgNPs showed antibacterial efficacy against clinical bacterial isolates of MDR in both Gram-negative, Streptococcus pneumoniae , and Gram-positive, Pseudomonas aeroginosa. By using the disk diffusion method. This activity might be attributed to the unique biological and physicochemical properties of the AgNPs, which facilitate the disruption of bacterial cell membranes, from results underscore the potential of Delftia acidovorans (OM838393.1) strain as an eco-friendly and efficient biological agent for the synthesis of AgNPs with potent antibacterial properties. The produced biosynthetic AgNPs revealed a significantly high-antioxidant activity compared to ascorbic acid in the same concentration. Conclusion: It can be concluded that AgNPs can be bio-synthesized using Delftia acidovorans (OM838393.1) strain bacteria in an eco-friendly way and these AgNPs can be used as a cost-effective antibacterial agent and antioxidant.