Molecular Identification of Providencia spp .in birds and their owners in Baghdad, Iraq

This study was conducted in different farms of birds to isolate Providencia spp from birds and their owners , A total of 100 samples were collected from different types of birds from cloacal swab of birds include pigeons (25), chickens (25) ,ducks(25), pet birds (15), parrots (10 ) collected from farms of bird and (50) stool samples from owners in different places in Baghdad city , Iraq. samples were inoculated in peptone water then cultured in Blood and MacConkeyagar. Identification of isolates was confirmed by conventional methods include (microscopic examination, cultural characteristic, biochemical tests), Vitek2, and 16S rRNA with sequencing. of 150 samples only five samples positive 5/150 (3.3%) and P. vermicola were the 2/5(0%), P. stuartii 3/5(60%) and number of isolate were in chickens 1/5(20%),in ducks 1/5(40%),in pet birds 1/5(20%) and in pigeons 1/5(20%) and in owners 1/5(20%).our results findings indicate that dropping of birds might be contaminated with Providencia spp.and this may leading to zoonosis transmission to all contact with birds causing infection like poisoning, diarrhea, urinary tract infections and other infections