Effect of Proteus Lipopolysaccharide (LPS) on virulence factor of Proteus mirabilis

Two hundred samples were collected from different samples (urine, ear and woundsswaps), from AL-Ramadi Teaching Hospital, Ramadi Maternity & Children Teaching Hospital.The results of cultural, microscopic, biochemical tests indicated that in urine samples K.pneumonia have high occurrence frequency 35 (29%) followed by Proteus mirabilis26(21.7%) andPseudomonas aeruginosa 20 (16.7%),while in wounds samples Pseudomonas aeruginosa10(25%)followed by Klebsiella pneumonia5 (12.5%) and Proteus mirabilis 3 (7.5%) , while Ear swaps samplesrevealed that P.aeruginosa 12(30%) was the major bacterium followed by P.mirabilis 4(10%)andKlebsiella pneumonia 2(5%) . Sensitivity test against twelve antimicrobial agents was done for allof the Proteus mirabilis isolates (33 isolates).The results displayed that most of the isolates wereresistant to Nitrofurantion (100%) ,Cefepime 70% followed by each Trimethoprim Sulfamethoxazole(60.6%) and Ceftriaxone(60.6%) while the most effective antimicrobial agent against P.mirabilis were Amoxicillin+Clavulanic acid (96.97%), Ofloxacin (78.79%),Levofloxacin (78.78 %) Ciprofloxacin (69.7%),Amikacin (66.7%), Imipenem (66.67%), Gentamicin (54.5%) and Naldixic acid (54.55%). Moreresistant isolate was selected, and lipopolysaccharide was extracted by hot Phenol method thismethod was tedious, but yielded LPS of high purity and the yield was (93) mg LPS from (5)g dryweight cell of P.mirabilis After partial purification, chemical analysis of crud and partial purifiedLPS showed that the amount of carbohydrate and protein in crude and partial purified LPSextract were (69.375)mg ml-1, and (7.107)mg ml-1) respectively followed by, while the Carbohydrate in dialysis (85975 mg ml-1) (1.21mg ml-1) and in partial purification of LPS (93.011mg ml-1) while protein 0.144mg ml-1.