Biomedical Applications of Chiropsoides buitendijki Crude Extract: Exploring Hemolytic, Anti-Angiogenic, and Neuromodulatory Properties for Therapeutic Potential

ABSTRACT: The research explores the biomedical properties of the box jellyfish Chiropsoides buitendijki, collected from Edwan village, Mumbai. The crude extract of the box jellyfish Chiropsoides buitendijki exhibits a range of bioactive properties with potential biomedical applications. Hemolytic activity observed in both human and chicken red blood cells indicates the presence of toxic proteins. The chick Chorio-Allantoic Membrane (CAM) assay revealed anti-angiogenic effects, including blood vessel damage and inhibition of neovascularization, suggesting potential use in cancer therapy. Neuromodulatory effects were assessed in Sprague-Dawley rat brains, where increased extract concentrations elevated Na⁺/K⁺-ATPase activity and cholinesterase inhibition, implicating potential impacts on metabolic stability and cognitive function. Protein analysis revealed a moderate protein content (0.121 mg/mL). Partial purification was achieved through SDS-PAGE electrophoresis, and the proteins were identified at various 11 kDa to 245 kDa levels, Functional characterization linked specific bands to hemolytic, antimicrobial, immunological, and anti-inflammatory activities. These findings underscore the pharmacological potential of Chiropsoides buitendijki extract, supporting its further exploration as a source of novel compounds for pharmaceutical and biomedical applications.