The Effect of Purwoceng (Pimpella alpina Molk.) on Oxidative Stress and Immunity in Wistar Rats

Exercise can significantly impact the body's redox status due to the generation of free radicals during physical activity. It also influences the regulatory mechanism of antioxidants. Purwoceng (Pimpella alpina Molk) is known for its high antioxidant properties and its ability to enhance the immune system. The phytochemical compound found in purwoceng provide protection against free radicals. This study investigated the effects of purwoceng on oxidative stress and immunity in Wistar rats subjected to high-intensity exercise. Thirty Wistar rats were randomly assigned to five groups (n = 6): a negative control (Group I), a positive control (Group II), and three treatmentgroups receiving purwoceng powder at doses of 36 mg/200 g BW/day (Group III), 72 mg/200 g BW/day (Group IV), and 108 mg/200 g BW/day (Group V). The treatment was administered once daily after exercise for 28 days. Malondialdehyde (MDA) level was assessed using ELISA, while lymphocytes count was measured from retroorbital blood samples. Data were analyzed using paired sample t-tests and one-way analysis of variance (ANOVA). The results indicated significant differences in MDA levels and lymphocytes counts before and after the administration of purwoceng powder at all tested doses (p < 0.05). Post hoc Bonferroni tests revealed differences in MDA and lymphocytes levels between the control and treatment groups. From the findings of the present study, it can be concluded that purwoceng powder effectively reduces MDA levels and enhances lymphocytes counts in Wistar rats subjected to high-intensity exercise.