Medial lethal concentration 50 (LC50) of a Chlorantraniliprole-based insecticide in Danio rerio

Chlorantraniliprole (CAP) is a widely used insecticide whose potential toxicity to non-target aquatic organisms remains a concern. This study evaluates the acute toxicity, developmental toxicity of a chlorantraniliprole-based insecticide in zebrafish (Danio rerio), a standard model for toxicological research. One-hour post-fertilization embryos were exposed to varying concentrations (7.4–74 µg/ml) to determine the median lethal concentration (LC50). The LC50 was 17.56 µg/ml, with dose-dependent mortality observed. Initial mortality was low during the first 72 hours but increased sharply post-hatching, indicating time-dependent toxicity mediated by the protective chorion. These findings reveal CAP’s emphasizing the need for stringent environmental monitoring of this insecticide.