Estimation of Added Urea in the Food Products and Marketed Formulation using 1,2-Napthoquinone-4-Sulphonate Reagent by UV- Visible Spectrophotometer

Milk naturally contains urea, which makes up a large portion of the amount of non-protein nitrogen found in the milk. The amount of urea in milk varies throughout herd members. Following that, urea and the Folin (NQS) solution mix to form a vibrant complex that is visible as an absorbed range of 454 nm. Following optimization, the limit of detection and quantification limitation were found to be the optimal experimental variables, at 0.00280 and 0.00848 µg/ml, respectively. The calibration plot for urea shows a straight line in a concentration that ranges from 0.1–10 µg/mL. The coefficient of the determination was R2 = 0.9999.