Phytochemical Analysis and Anti-Urolithiatic Potential of Cyamopsis tetragonoloba and Trichosanthes dioica

Objective: The medicinal and industrial uses of plants have long been acknowledged. Bioactive chemicals, nutritional advantages, and pharmacological characteristics are what make Cluster Bean (Cyamopsis tetragonoloba) and Pointed Gourd (Trichosanthes dioica) so special. The purpose of this research is to determine whether or not they have antiurolithiatic properties and to examine their phytochemical makeup. Materials and methods: After collecting and verifying the plant materials, they were extracted using the Soxhlet apparatus and solvents such as petroleum ether and ethyl acetate. To find the most important bioactive chemicals, phytochemical screening was performed. For the purpose of phytochemical profiling, HPTLC and Thin Layer Chromatography (TLC) were employed. The anti-urolithiatic action was assessed in Wistar rats that had hyperoxaluria induced by ethylene glycol. Lactate dehydrogenase (LDH) activity, kidney oxalate levels, and urine parameters were examined. Results: Essential oils, glycosides, tannins, flavonoids, and saponins were all identified through phytochemical research. In terms of extraction efficiency, ethyl acetate extract outperformed petroleum ether. Several bioactive substances were found through chromatographic analysis. Treatment groups showed markedly enhanced renal function and reduced oxalate deposition in in vivo investigations, suggesting substantial anti-urolithiatic activity. Conclusion: The study highlights the potential use of C. tetragonoloba and T. dioica in nutraceutical and pharmaceutical applications by establishing their pharmacological relevance. To learn more about how effective they are as a treatment, additional studies involving clinical trials and formulation development are needed.