A Novel RP-HPLC Analytical Method Development and Validation of Berberine In Bulk and Polyherbal Formulation

A simple, precise, accurate and reproducible method for the estimation of Berberine in bulk and polyherbal formulations by RP-HPLC. The separation of the phytochemical using RP-HPLC was achieved on a SHISEIDO CAPSELL PAK C18, (250×4.6mm, 5 micron) size column with a mobile phase consisting Acetonitrile : 0.1% Orthophosphoric acid (60:40), using a flow rate of 1 mL/min and detecting a wavelength of 265 nm.The retention times was observed to be 2.678 for Berberine respectively. Linearity was found to be 2, 4, 6, 8, 10μg/ml for Berberine, with correlation of coefficient of 0.996 respectively. For system precision the %RSD for Berberine was found to be less than 2.0. the developed method was succesfully validated for Accuracy, Precision, Linearity,Limit of Detection, Limit of Quantification & Robustness. Hence, this method can be used for estimation of Berberine in bulk and polyherbal formulations by RP-HPLC.