Engineering of the Caspase-3 Gene in Recombinant CHO Cells Caused More Apoptosis Resistance and enhanced Recombinant Protein Production Than the BAX Gene.
Our observations suggest that the downregulation of the BAX and caspase-3 genes using the CRISPR method, inhibits apoptosis and enhances the yield of recombinant EPO, even in the presence of an apoptosis inducer. Additionally, reduction of caspase-3 expression was proved to be more effective than BAX in extending the lifespan of cells and producing heterologous recombinant proteins.
Authors
- Saadi Hosseini (ORCID: https://orcid.org/0000-0002-2411-7903)
- Hooman Kaghazian (ORCID: https://orcid.org/0000-0003-4301-3381)
- Hosein Shahsavarani (ORCID: https://orcid.org/0000-0002-8123-0857)
- Morteza Karimipoor (ORCID: https://orcid.org/0000-0002-2406-5963)
- Reza Mahdian (ORCID: https://orcid.org/0000-0003-1458-4767)
- A Rahimi (ORCID: https://orcid.org/0000-0002-1982-8081)
- Atefeh Alipour (ORCID: https://orcid.org/0000-0003-1837-4150)
- Mohammad Ali Shokrgozar (ORCID: https://orcid.org/0000-0002-9198-4070)
- Marzieh Mohammadi (ORCID: https://orcid.org/0000-0002-7026-4840)
Institutions
- Pasteur Institute of Iran (IR)
- Shahid Beheshti University (IR)
Publication Details
- Journal
- PubMed
- Published
- 2025-05-01
- DOI
- https://doi.org/10.61186/ibj.4934
- Primary Topic
- Viral Infectious Diseases and Gene Expression in Insects
- Type
- article
- Field-Weighted Citation Impact
- 0.00