Fibrinolytic Activity of Enzyme Extract of Pucuk Merah (Syzygium myrtifolium Walp.) Phosphate Buffer Solvent using Fibrin Plate and Clot Lysis Methods

The fibrinolytic protease enzyme is an enzyme that has the ability to break down blood clots that can cause cardiovascular disease. This enzyme can be found in pucuk merah (Syzygium myrtifolium Walp), which is a popular plant and easy to find. The aim was to determine the total protein content, protein molecular weight, and fibrinolytic activity of pucuk merah leaf enzyme extract which were tested using fibrin plate and clot lysis methods. The stages of the research included enzyme extraction using 0.1 M phosphate buffer pH 7, precipitation of crude enzyme extract with 80% ammonium sulfate, dialysis of enzyme extract using 12-14 kDa cut-off dialysis bags, enzyme extract characterization using SDS-PAGE, total protein measurement using the Bradford method and testing the fibrinolytic activity of enzyme extracts using the fibrin plate and clot lysis methods. Positive and negative controls are nattokinase and phosphate buffer. The fibrinolytic activity of the enzyme extract was analyzed by one-way analysis of variance (ANOVA) using SPSS 26 software. The highest total protein content was in the enzyme extract precipitate sample of 1864 μg/ml. The molecular weight of the fibrinolytic enzyme extract of pucuk merah leaves ranges from 25 to 45 kDa. The precipitate sample of pucuk merah leaf enzyme extract had the highest fibrinolytic activity against fibrin plate testing of 11.23 mm and clot lysis testing of 13.90%. Enzyme extract precipitate is the most effective sample because it has the highest fibrinolytic activity. Keywords: clot lysis, fibrinolytic, fibrin plate, pucuk merah