Antioxidant, Photoprotectitive and Antiinflammatory activity of Okra (Abelmoschus esculentus L.) Fruit Extract

Abelmoschus esculentus L. is a vegetable that contains secondary metabolites. This study tested antioxidant activity with IC50 parameters, the ability of this extract as a sunscreen with SPF, erythema and pigmentation as parameters, and an anti-inflammatory test. Abelmoschus esculentus fruit was extracted with 80% ethanol and obtained a yield of 30.68%. Screening phytochemicals to prove that Abelmoschus esculentus fruit extract contains saponins, flavonoids, steroids, tannins, and alkaloids. Compounds that have the potential to be antioxidants, sunscreens and anti-inflammation agents are phenols, especially flavonoids, so the quantitative amount is analyzed. The total amount of phenolic compounds in Abelmoschus esculentus fruit extract was 113.4751 mgQA/g, and the total amount of flavonoids was 61.1284 mgQE/g. For testing antioxidant activity with the DPPH method, the IC50 was obtained at 53.5303 mcg/mL, which is a strong category. As for testing sunscreen, the SPF value of ethanol extract in vitro using a UV-Vis spectrophotometer at levels of 100 mcg/mL of 1.1295 and 200 mcg/mL of 1.5748 means that concentration has not been able to become a sunscreen because the SPF value is still below the minimum protection category, but 400 mcg/mL levels of 2.2610 can provide minimal protection. The results of the anti-inflammatory test gave results of IC50 is 153.837 mcg/L.